GUESS PAPER - 2008

CLASS - XII

SUBJECT – BIOTECHNOLOGY

(SET-3)

Time Allowed: 3 Hours Maximum Marks: 70

SECTION A (1 marks each)

1. Why sickle celled anemia is called a molecular disease?

2. Give one characteristics feature of type-II restriction enzyme.

3. Name any two molecular markers.

4. What kind of data can be accessed from the data retrieval tool called

Taxonomic browser?

5. Give the term which can be used for the organ grafts transferred

between a donor & a recipient.

SECTION B ( 2 marks each)

6. Give the advantage and disadvantage of serum containing media.

7. What are the factors governing the stability of karyotype?

8. From a given group of crop plants how will you diagnose the

diseased plants? Explain any one technique.

9. From the given table determine which organism has maximum

doubling time and why?

Microorganisms specific growth

rate (h)

Aspergillus nidulans 0.36

Penicillium chrysogenum 0.12

Benechean atriegans 4.24

Methylomonas methenolytica 0.12

10. Name the device commonly used for continuous culture technique.

Name the two factors that control the working of a chemostat.

11. Expand the term BLAST .Give the common use of the following

programmers (i) BLASTp (ii)TBLASTn.

12. Which vectors are used in human genome sequencing & why?

13. Explain the blue white method of screening the recombinant

plasmid?

14. A single cell of E.coli is supposed to produce about 2000 different

proteins.

15. under optimum conditions. One of the desired intercellular enzymes

produced is in the form of 2500 molecules/cells. If the molecular

weight of that enzyme is 100000D.How many E.coli cells are required

to produce 1gm of enzymes.

16. What are secondary metabolites? Name the secondary metabolites

produced by the following plants. (a)Atropa beladona (b) Cinchona

officinalis.

SECTION C (3 marks each)

16.What is the principle behind the working of a mass spectrometer?

Explain the various components of it.

17.Define essential amino acids. What is the importance of BCAA in

athletes. Which protein has highest amount of BCAA.

18. With the help of schematic explain the method for constructing a

cDNA library .What are the advantage of cDNA over genomic DNA?

19. Give at least five uses of bioinformatics tools in analysis of biological

data.

20. Who pioneered the dideoxy chain termination method .What are its

requirements .Explain the procedure in detail.

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